منابع مشابه
Polarity of spindle microtubules in Haemanthus endosperm
Structural polarities of mitotic spindle microtubules in the plant Haemanthus katherinae have been studied by lysing endosperm cells in solutions of neurotubulin under conditions that will decorate cellular microtubules with curved sheets of tubulin protofilaments. Microtubule polarity was observed at several positions in each cell by cutting serial thin sections perpendicular to the spindle ax...
متن کاملDecoration of spindle microtubules with Dynein: evidence for uniform polarity
Studies were conducted to determine whether the microtubules present within native spindles isolated from eggs of the surf clam, Spisula solidissima, could bind dynein obtained from axonemes of Tetrahymena thermophila. SDS gel electrophoresis revealed that the high molecular weight polypeptides that make up dynein cosedimented with the isolated spindles. Moreover, the ATPase activity of dynein ...
متن کاملSpindle microtubules in flux.
Accurate and timely chromosome segregation is a task performed within meiotic and mitotic cells by a specialized force-generating structure--the spindle. This micromachine is constructed from numerous proteins, most notably the filamentous microtubules that form a structural framework for the spindle and also transmit forces through it. Poleward flux is an evolutionarily conserved mechanism use...
متن کاملCortical capture of microtubules and spindle polarity in budding yeast - where's the catch?
In asymmetric divisions, the mitotic spindle must align according to the cell polarity axis. This is achieved through targeting astral microtubules emanating from each spindle pole to opposite cell cortex compartments. Saccharomyces cerevisiae is a powerful genetic model for dissection of this complex process. Intense research in this yeast has rendered detailed models for a program linking act...
متن کاملStructural Polarity of Kinetochore Microtubules
The polarity of kinetochore microtubules (MTs) has been studied in lysed PtK1 cells by polymerizing hook-shapdd sheets of neurotubulin onto the walls of preexisting cellular MTs in a fashion that reveals their structural polarity . Three different approaches are presented here : (a) we have screened the polarity of all MTs in a given spindle cross section taken from the region between the kinet...
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ژورنال
عنوان ژورنال: Nature
سال: 1980
ISSN: 0028-0836,1476-4687
DOI: 10.1038/284402b0